Composite

Part:BBa_M1021:Design

Designed by: Miguel Suastegui   Group: UtahState_BE5930_-_S11   (2011-04-12)


RBS+ "Blues" Zinc Finger + PhaC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1880
    Illegal AgeI site found at 111
    Illegal AgeI site found at 198
    Illegal AgeI site found at 342
    Illegal AgeI site found at 615
    Illegal AgeI site found at 1398
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequences were fused using the Silver fusion standard. Both sequences are optimized for expression in E. coli


Source

PhaC: A.eutrophus poly-beta-hydroxybuterate-C (phbC) gene

References